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NIGER DELTA JOURNAL OF MEDICINE & MEDICAL RESEARCH

A Publication Of Nigeria Medical Association Bayelsa State Branch

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Archive | ISSUE: 131, Volume: Jan-Mar-2025

IDENTIFICATION AND CHARACTERIZATION OF PSEUDOMONAS SPECIES IN WOUND SAMPLES COLLECTED FROM FEDERAL MEDICAL CENTER (FMC), YENAGOA, BAYELSA STATE, NIGERIA


Author:Pughikumo DT, Alade T2, Sylvanus J

published date:2025-Mar-16

FULL TEXT in - | page 23- 29

Abstract

Background: Pseudomonas species are Gram-negative, motile rods bacteria which are motile rod-like in shape and capable of widely being distributed in diverse environments with P. aeruginosa species notably effecting humans.Phenotypic identification of Pseudomonas species is less reliable, time consuming and not 100 percent accurate due to errors made by scientists and accurate identification and characterization of Pseudomonas species is essential for appropriate selection of antibiotics for treatment. Molecular technique particularly 16SrRNA gene amplification and sequencing is a more reliable accurate and precise tool been introduced for pseudomonas aeruginosa.

Methodology: A prospective study aimed at the identification and characterization of Pseudomonas species in wound samples collected from patients in Federal Medical Center (FMC), Yenagoa was carried out. Of the 50 wound samples collected, identified and characterized using standard phenotypical and 16SRNA amplification techniques.

Results: The distribution was as follows; age range was between 11- 60years, 38(64%) samples yielded no growth and 18 (34%) yielded growth, of which 7(39%) were Pseudomonas aeruginosa and others isolated were Staphylococcus spp’, Escherichia coli and Kiebsieila spp. The isolates of Pseudomonas aeruginosa analyzed statistically, showed no statistical difference. The molecular characterization confirmed the identity of the organism as of phenotypic features.

Conclusion: Molecular characterization appears to be more accurate and precise than the phenotypic method. It is recommended that Government should provide molecular diagnostic tools and equipment’s in the health care system to help in accurate diagnosis and identification of bacteria and also fund the training of relevant Scientists on molecular techniques for easy diagnosis.

Key Words: Pseudomonas spp., Molecular characterization, 16SRNA amplification

Keywords: Pseudomonas spp., Molecular characterization, 16SRNA amplification,,

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